1Sf1Ep mediumCulturing Treponema pallidum, the bacteria that causes syphilis, in vitro is a challenge. T. pallidum is not routinely cultured in the laboratory for diagnostic purposes, in part because it cannot be grown on artificial media. However, a breakthrough in culturing T. pallidum was reported in 2018 when researchers managed to grow the bacterium in a rabbit epithelial cell line (Sf1Ep) using a medium called 'Sf1Ep medium'. It's not used for routine diagnostic purposes, but for research only. The diagnosis involves direct microscopic examination, serologic tests, molecular tests and histopathology.
1Petragnini MediumAn egg-based medium enriched with additional nutrients to promote the growth of Mycobacterium tuberculosis. Colonies of M. tuberculosis are small, round, buff-colored, and typically take 3-4 weeks to appear.
1CHROMagar CandidaThis differential medium allows for the isolation and identification of Candida species based on colony color. Candida albicans usually forms green colonies on this medium.
1Liquid Media (such as Nutrient Broth)E. coli exhibits homogenous turbid growth within 12-18 hours. After prolonged incubation, pellicles may form on the surface of the media.
1Dubos’ MediumA liquid medium that contains a mixture of salts, fatty acids, and polysorbate. When M. tuberculosis grows in this medium, it causes the medium to become turbid.
2Pawlowsky MediumA potato-based medium. Growth of M. tuberculosis may be similar to that seen on the LJ Medium.